2OVE
Crystal Structure of Recombinant Human Complement Protein C8gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.227, 59.775, 71.901 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.000 |
| Rwork | 0.226 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.148 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.114 |
| Number of reflections | 12610 | |
| <I/σ(I)> | 12.6 | 8.5 |
| Completeness [%] | 97.8 | 95 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 0.1 M sodium citrate and 25-27% PEG 4000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
