2OVD
Crystal Structure of Human Complement Protein C8gamma with Laurate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91963 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.081, 59.539, 70.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 1.800 |
| Rwork | 0.208 |
| R-free | 0.23000 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.153 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.058 | 0.335 |
| Number of reflections | 18059 | |
| <I/σ(I)> | 10.3 | 3.7 |
| Completeness [%] | 97.0 | 77.4 |
| Redundancy | 6.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 0.1 M sodium citrate and 25-27% PEG 4000. Sodium laurate (Sigma) was solubilized in methanol/water and used to prepare a 0.9 mM solution in 0.1M imidazole containing 32% PEG 4000, pH 7.0, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
