2OV9
Crystal structure of protein RHA08564, thioesterase superfamily protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.705, 70.132, 81.511 |
| Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
| Resolution | 38.800 - 1.900 |
| R-factor | 0.19611 |
| Rwork | 0.194 |
| R-free | 0.23632 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.537 |
| Number of reflections | 60918 | |
| <I/σ(I)> | 33.9 | 2.21 |
| Completeness [%] | 98.1 | 82.2 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M Lithium sulfate, 0.1M Bis-Tris pH 6.5, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
