2OUH
Crystal structure of the Thrombospondin-1 N-terminal domain in complex with fractionated Heparin DP10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.316, 41.069, 241.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.257 |
| Rwork | 0.253 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z78 |
| RMSD bond angle | 0.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.046 | 0.268 |
| Number of reflections | 14771 | |
| <I/σ(I)> | 29.87 | 3.63 |
| Completeness [%] | 88.8 | 65.9 |
| Redundancy | 4.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 30% PEG1500, 0.08M Sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
