2OU8

Structure of Spin-labeled T4 Lysozyme Mutant T115R1 at Room Temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2002-05-17
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	60.945, 60.945, 97.257
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.19
Rwork	0.187
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzm
RMSD bond length	0.007
RMSD bond angle	0.023
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.350
Number of reflections	19612
<I/σ(I)>	14	2.8
Completeness [%]	98.4	98.4
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	296	1.8 M Na/K Phosphate, 240 mM NaCl, 40 mM 2-hydroxyethyl disulfide, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 296K