2OU3

Crystal structure of a tellurite resistance protein of cog3793 (npun_f6341) from nostoc punctiforme pcc 73102 at 1.85 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.911625, 0.979310, 0.979051
Spacegroup name	P 21 21 21
Unit cell lengths	38.490, 56.190, 152.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.311 - 1.850
R-factor	0.163
Rwork	0.161
R-free	0.21300
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.311	29.310	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.087	0.033	0.415
Number of reflections	29051
<I/σ(I)>	8.31	21.05	1.99
Completeness [%]	99.5	98.8	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 10.0% 2-propanol, 0.2M Zn(OAc)2, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K