2OTB
Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.016, 67.736, 95.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zux chain A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.073 | 0.176 |
| Number of reflections | 40185 | |
| <I/σ(I)> | 14.8 | 21.1 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 31% PEG 3400, 0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 2.9 mM 1-s-nonyl-beta-D-thioglucoside, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
