2OT7

Crystal structure of JMJD2A complexed with histone H3 peptide monomethylated at Lys9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0723
Spacegroup name	P 21 21 2
Unit cell lengths	101.209, 149.842, 57.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.135
R-factor	0.18909
Rwork	0.187
R-free	0.24072
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2oq7
RMSD bond length	0.012
RMSD bond angle	1.330
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.920	2.250
High resolution limit [Å]	2.135	2.135
Rmerge	0.072	0.541
Number of reflections	48784
<I/σ(I)>	11.9	2
Completeness [%]	99.1	100
Redundancy	2.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	20% PEG 3350, 100 mM Citrate, 2 mM NiCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K