2OS9
crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with myoinositol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.564, 108.692, 55.774 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 27.870 - 1.700 |
| R-factor | 0.19724 |
| Rwork | 0.195 |
| R-free | 0.22274 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2ggu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.900 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 69027 | |
| <I/σ(I)> | 23.4 | 4 |
| Completeness [%] | 94.7 | 87.7 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 150mM NaCl, 10mM CaCl2, 12% PEG 8000, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
