2OS5

Macrophage migration inhibitory factor from Ancylostoma ceylanicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-17
Detector	ADSC QUANTUM 315
Spacegroup name	P 63 2 2
Unit cell lengths	115.340, 115.340, 199.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 1.600
R-factor	0.19532
Rwork	0.195
R-free	0.20016
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.241
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.600
Rmerge	0.116
Number of reflections	88266
<I/σ(I)>	3.8
Completeness [%]	90.0
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2.0 M ammonium sulfate, 0.1 M sodium citrate, 0.2 M potassium sodium tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2.0 M ammonium sulfate, 0.1 M sodium citrate, 0.2 M potassium sodium tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2.0 M ammonium sulfate, 0.1 M sodium citrate, 0.2 M potassium sodium tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K