2OS3
Structures of actinonin bound peptide deformylases from E. faecalis and S. pyogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-22 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 1.12714 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 40.557, 40.557, 217.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.940 - 2.260 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lqy |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.095 | 0.381 |
Number of reflections | 13084 | |
<I/σ(I)> | 26.7 | 3 |
Completeness [%] | 98.1 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 30% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |