2ORX

Structural Basis for Ligand Binding and Heparin Mediated Activation of Neuropilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-09
Detector	MAR CCD 165 mm
Wavelength(s)	1.38
Spacegroup name	P 21 21 21
Unit cell lengths	38.094, 92.221, 120.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.370 - 2.400
R-factor	0.20402
Rwork	0.201
R-free	0.25178
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kex
RMSD bond length	0.011
RMSD bond angle	1.405
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	16862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	100mM HEPES pH 7.6, 10% PEG 20K, 7% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K