2OQ5
Crystal structure of DESC1, a new member of the type II transmembrane serine proteinases family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-21 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.900, 70.200, 80.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.610 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Enteropeptidase |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.790 | |
Number of reflections | 31409 | |
Completeness [%] | 86.3 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris , 8 % (m/w) PEG 8000 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |