2OQ2
Crystal structure of yeast PAPS reductase with PAP, a product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97944 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.418, 63.082, 323.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.24600 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.178 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.833 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.073 | 0.373 |
Number of reflections | 62580 | |
<I/σ(I)> | 7.2 | 1.6 |
Completeness [%] | 96.5 | 78.2 |
Redundancy | 4.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% w/v PEG 6000, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% w/v PEG 6000, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% w/v PEG 6000, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% w/v PEG 6000, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |