Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.240, 73.874, 68.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.20172 |
| Rwork | 0.198 |
| R-free | 0.24713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k9u |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 37132 | |
| <I/σ(I)> | 18.1 | 3.2 |
| Completeness [%] | 97.9 | 84.5 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.4 | 293 | 0.2 M ammonium sulfate, 22% PEG 4000, 0.1 M alanine/HCl, pH 3.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
