2OPL

Crystal structure of an osmc-like protein (gsu2788) from geobacter sulfurreducens at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97894, 0.97870
Spacegroup name	P 21 21 21
Unit cell lengths	47.240, 83.010, 95.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.500
R-factor	0.151
Rwork	0.150
R-free	0.16800
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.487
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.710		1.550
High resolution limit [Å]	1.500	2.560	1.500
Rmerge	0.048	0.036	0.311
Number of reflections	60730
<I/σ(I)>	11.02	21.4	2.37
Completeness [%]	99.5	99.3	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	NANODROP, 0.2M (NH4)2SO4, 25.0% PEG 4000, 0.1M Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K