2OPI
Crystal Structure of L-fuculose-1-phosphate aldolase from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.934, 115.934, 73.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.550 - 2.500 |
| R-factor | 0.22667 |
| Rwork | 0.225 |
| R-free | 0.25955 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.745 |
| Number of reflections | 19838 | |
| <I/σ(I)> | 23.81 | 1.67 |
| Completeness [%] | 98.4 | 92.5 |
| Redundancy | 9.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.2 M Ammonium sulfate, 0.1 M Sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
