2OPG
The crystal structure of the 10th PDZ domain of MPDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 54.944, 54.944, 118.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.470 - 1.500 |
| R-factor | 0.17473 |
| Rwork | 0.172 |
| R-free | 0.21718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fne |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.929 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.455 |
| Number of reflections | 33890 | |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M BIS-TRIS, 20% mPEG 5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
