2OOY
Crystal structure of the adenylate sensor from AMP-activated protein kinase complexed with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.902, 77.666, 107.576 |
| Unit cell angles | 90.00, 123.99, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.880 |
| R-factor | 0.1946 |
| Rwork | 0.189 |
| R-free | 0.29784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oox |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.100 | 0.417 |
| Number of reflections | 24969 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 96.2 | 93.4 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7.3-8.1% PEG3350, 0.1M HEPES, pH 7.5, 5mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
