2OOX
Crystal structure of the adenylate sensor from AMP-activated protein kinase complexed with AMP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97898, 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.456, 97.392, 168.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.20975 |
Rwork | 0.206 |
R-free | 0.27348 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.804 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.098 | 0.469 |
Number of reflections | 38081 | |
<I/σ(I)> | 20.1 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 6.2-7.2% PEG6000, 10% Ethylene glycol, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |