2OOF
The crystal structure of 4-imidazolone-5-propanoate amidohydrolase from environmental sample
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.851, 95.851, 115.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.750 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.116 | 0.503 |
| Number of reflections | 30852 | |
| <I/σ(I)> | 10 | 9.7 |
| Completeness [%] | 97.2 | 81.4 |
| Redundancy | 18.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.8 M Tri-Ammonium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
