2OOE
Crystal structure of HAT domain of murine CstF-77
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.675, 105.675, 358.157 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.170 - 3.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (THEN SOLVE/RESOLVE) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.071 | 0.199 |
| Number of reflections | 15612 | |
| <I/σ(I)> | 16.1463 | 5.691 |
| Completeness [%] | 98.1 | 99.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 50mM Bis-tris, 50mM ammonium sulfate, 30%(v/v) pentaerythritol ethoxylate(15/4EO/OH), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
