2OO6
Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.906, 104.906, 145.658 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.130 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.109 | 0.825 |
| Total number of observations | 7055 | |
| Number of reflections | 35232 | |
| <I/σ(I)> | 16.8 | 0.9 |
| Completeness [%] | 93.2 | 56.8 |
| Redundancy | 8.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 100mM Tris-HCl pH 8.5, 12% Glycerol, 1.5M Ammonium sulfate, VAPOR DIFFUSION, temperature 294K |
