2ONU
Plasmodium falciparum ubiquitin conjugating enzyme PF10_0330, putative homologue of human UBE2H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.821, 52.821, 206.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.380 |
| R-factor | 0.21528 |
| Rwork | 0.213 |
| R-free | 0.24978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YH6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.102 | 0.961 |
| Number of reflections | 12626 | |
| <I/σ(I)> | 7.5 | 2.41 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25% PEG 3350, 0.1 M Ammonium sulfate, 0.1 M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
