2ONF
Crystal structure of a putative osmotically inducible protein c (ta0195) from thermoplasma acidophilum at 1.70 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.070, 68.550, 48.670 |
| Unit cell angles | 90.00, 98.29, 90.00 |
Refinement procedure
| Resolution | 27.929 - 1.700 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.696 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.929 | 1.760 | |
| High resolution limit [Å] | 1.700 | 2.900 | 1.700 |
| Rmerge | 0.032 | 0.021 | 0.315 |
| Number of reflections | 33417 | ||
| <I/σ(I)> | 14.34 | 30 | 2.6 |
| Completeness [%] | 94.4 | 97.3 | 87.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | NANODROP, 30.0% PEG-6000, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
