2ONE
ASYMMETRIC YEAST ENOLASE DIMER COMPLEXED WITH RESOLVED 2'-PHOSPHOGLYCERATE AND PHOSPHOENOLPYRUVATE
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.100, 110.000, 66.200 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.000 |
| R-factor | 0.137 * |
| Rwork | 0.137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | YEAST ENOLASE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.056 |
| Total number of observations | 96445 * |
| Number of reflections | 29854 |
| Completeness [%] | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | pH 7.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 50 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | ammonium sulfate | 55 (%sat) | |
| 7 | 1 | reservoir | ammonium sulfate | 55 (%sat) |
