2OMO
Putative antibiotic biosynthesis monooxygenase from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.155, 99.248, 192.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.740 - 1.830 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.22770 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.740 | 1.910 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.094 | 0.762 |
| Number of reflections | 82843 | |
| <I/σ(I)> | 29.7 | 2.19 |
| Completeness [%] | 95.9 | 57.8 |
| Redundancy | 13.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Sodium chloride, 0.1 M Bis-Tris buffer, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
