2OMD

Crystal structure of molybdopterin converting factor subunit 2 (aq_2181) from aquifex aeolicus VF5

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-15
Detector	RIGAKU RAXIS V
Wavelength(s)	0.9788, 0.9000, 0.9794
Spacegroup name	P 61 2 2
Unit cell lengths	119.995, 119.995, 125.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.890 - 2.000
R-factor	0.19
Rwork	0.190
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.067	0.249
Number of reflections	36659
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	10% 1,2-propanediol, 25% Glycerol, MICROBATCH, temperature 291K