2OLO
NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.710, 90.570, 85.400 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.184 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.200 |
| Number of reflections | 43421 | |
| <I/σ(I)> | 29.8 | 5 |
| Completeness [%] | 92.8 | 60.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 100 mM MES buffer, 25-30% 2-methyl-2,4-pentanediol, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
