2OLH

Crystal structure of a signalling protein (SPG-40) complex with cellobiose at 2.78 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2007-01-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54132
Spacegroup name	P 21 21 21
Unit cell lengths	62.690, 66.450, 107.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.780
R-factor	0.191
Rwork	0.191
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ljy
RMSD bond length	0.009
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.940	2.880
High resolution limit [Å]	2.780	2.780
Number of reflections	11536
Completeness [%]	99.5	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	Tris-HCl, Ethanol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K