2OLA
Crystal structure of O-succinylbenzoic acid synthetase from Staphylococcus aureus, cubic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 144.773, 144.773, 144.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.21276 |
| Rwork | 0.211 |
| R-free | 0.26081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wue |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.068 | 0.580 |
| Number of reflections | 18662 | |
| <I/σ(I)> | 7.1 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.6 | 22.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.8M Sodium malonate, PH 7.0, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
