2OKZ
MVGGVV peptide derived from Alzheimer's A-beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-16 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 15.148, 9.576, 23.732 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 23.560 - 1.800 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MVGGVV from space group P1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.685 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.158 | 0.447 |
| Number of reflections | 650 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 95.3 | 89.8 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.2 M CaCl2, 20% isopropanol, 0.1 M Na acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
