2OKY
A non-invasive GFP-based biosensor for mercury ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979454 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.240, 87.240, 119.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.400 |
| R-factor | 0.2251 |
| Rwork | 0.225 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1emb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.824 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.936 | 2.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.036 | 0.149 |
| Number of reflections | 18556 | |
| <I/σ(I)> | 38.46 | 9.99 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 14.4 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 32% PEG 8000, 100mM PIPES, 200mM ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
