2OKW

A non-invasive GFP-based biosensor for mercury ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	150
Detector technology	CCD
Collection date	2006-06-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979454
Spacegroup name	P 21 21 21
Unit cell lengths	88.178, 128.843, 147.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 1.900
Rwork	0.232
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emb
RMSD bond length	0.005
RMSD bond angle	1.374
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.830
High resolution limit [Å]	1.900
Rmerge	0.073
Number of reflections	155659
<I/σ(I)>	15.7
Completeness [%]	99.7
Redundancy	7.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM Pipes, 30% PEG 8000, 200mM Na-acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K