2OKN
Crystal Strcture of Human Prolidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.890, 108.960, 212.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.940 - 2.450 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iw2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 2.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.041 | 0.140 |
| Number of reflections | 44429 | |
| <I/σ(I)> | 26.2 | 9.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.14 | 4.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.5M sodium/potassium phosphate, 0.05M sodium chloride, 0.02% sodium acide, 0.02M dithiothreitol, 0.02M manganese chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
