2OK7

Ferredoxin-NADP+ reductase from Plasmodium falciparum with 2'P-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.931
Spacegroup name	P 31
Unit cell lengths	123.129, 123.129, 133.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.26303
Rwork	0.260
R-free	0.32083
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jb9
RMSD bond length	0.009
RMSD bond angle	1.412
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.102	0.476
Number of reflections	61398
<I/σ(I)>	9.8	2.3
Completeness [%]	98.5	98.1
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	293	PEG 4000 25%, buffer Na cacodylate, Na acetate 0.2 M, pH 6., microbatch, temperature 293K