2OK2
MutS C-terminal domain fused to Maltose Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.289, 88.361, 61.353 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.20501 |
| Rwork | 0.201 |
| R-free | 0.27725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fqc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.914 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 59119 | |
| <I/σ(I)> | 47 | 3.2 |
| Completeness [%] | 97.9 | 95.6 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 15% PEG 4K, 100 mM sodium citrate, 100 mM lithium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
