2OK0
Fab ED10-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 298 |
| Wavelength(s) | 1.427 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.711, 63.833, 83.880 |
| Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
| Resolution | 40.490 - 1.890 |
| R-factor | 0.17825 |
| Rwork | 0.177 |
| R-free | 0.20358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.856 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 44585 | |
| <I/σ(I)> | 17.2 | 2 |
| Completeness [%] | 94.5 | 91.5 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG 6000, 0.05M KCl, 0.01M MgCL2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCL2 | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | PEG 6000 | ||
| 6 | 1 | 2 | KCl | ||
| 7 | 1 | 2 | MgCL2 | ||
| 8 | 1 | 2 | HOH |
