2OK0
Fab ED10-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 298 |
Wavelength(s) | 1.427 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.711, 63.833, 83.880 |
Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
Resolution | 40.490 - 1.890 |
R-factor | 0.17825 |
Rwork | 0.177 |
R-free | 0.20358 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.856 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 44585 | |
<I/σ(I)> | 17.2 | 2 |
Completeness [%] | 94.5 | 91.5 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG 6000, 0.05M KCl, 0.01M MgCL2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | MgCL2 | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | PEG 6000 | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | MgCL2 | ||
8 | 1 | 2 | HOH |