2OJZ
Anti-DNA antibody ED10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 298 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.899, 194.045, 61.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.240 - 2.730 |
| R-factor | 0.20423 |
| Rwork | 0.201 |
| R-free | 0.26438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ggi |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.926 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 2.800 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Number of reflections | 27701 | |
| <I/σ(I)> | 16 | 3.7 |
| Completeness [%] | 97.9 | 97 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M ammonium sulfate, 0.1M sodium cacodylate, 25% MPEG 2000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.K |
