2OJQ
Crystal structure of Alix V domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795, 0.9799, 0.9600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.650, 55.380, 113.166 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 113.230 - 2.870 |
| R-factor | 0.2566 |
| Rwork | 0.250 |
| R-free | 0.31844 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.067 | 0.317 |
| Number of reflections | 10483 | |
| <I/σ(I)> | 17.1 | 3.1 |
| Completeness [%] | 97.1 | 86.6 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 13-15% PEG 6000, 6-10% ethylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
