2OIY
Crystal structure of the duplex form of the HIV-1(LAI) RNA dimerization initiation site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.963, 47.550, 57.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.25000 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 16259 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | MAGNESIUM CHLORIDE, CACODYLATE, POTASSIUM CHLORIDE, SPERMINE, MPD, pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MAGNESIUM CHLORIDE | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | CACODYLATE | ||
| 3 | 1 | 1 | POTASSIUM CHLORIDE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MAGNESIUM CHLORIDE | ||
| 8 | 1 | 2 | CACODYLATE | ||
| 9 | 1 | 2 | POTASSIUM CHLORIDE |
