2OIX
Xanthomonas XopD C470A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-24 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.953, 91.953, 45.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.330 - 1.800 |
| R-factor | 0.21191 |
| Rwork | 0.210 |
| R-free | 0.25903 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2oiv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.330 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.580 |
| Number of reflections | 17346 | |
| <I/σ(I)> | 30.6 | 1.95 |
| Completeness [%] | 94.2 | 77.4 |
| Redundancy | 7.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15 mg/mL protein in 20 mM Tris-HCl pH 7.5, 75 mM KCl, and 0.5 mM DTT and 1.4 - 1.6 M sodium potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
