2OIL
Crystal structure of human RAB25 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-03 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.250, 90.250, 45.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.537 - 2.300 |
| Rwork | 0.208 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oix |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.474 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.146 | 0.079 | 0.754 |
| Number of reflections | 8824 | 980 | 860 |
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 12.8 | 11.7 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% peg-3350, 0.2M ammonium chloride, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP |
