2OIJ
HIV-1 subtype B DIS RNA extended duplex AuCl3 soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.560, 47.460, 57.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.310 |
| Rwork | 0.272 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 10114 | |
| <I/σ(I)> | 43.1 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | NaCacodylate pH7.0, MgCl2, KCl, MPD, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | NaCacodylate pH7.0, MgCl2, KCl, MPD, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCacodylate | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaCacodylate | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MPD |
