2OI6
E. coli GlmU- Complex with UDP-GlcNAc, CoA and GlcN-1-PO4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 125 |
Detector technology | CCD |
Collection date | 2001-02-09 |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 2 |
Unit cell lengths | 102.760, 102.760, 644.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.960 - 2.200 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hv9 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.960 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.065 | 0.308 |
Number of reflections | 67353 | |
Completeness [%] | 98.0 | 93.9 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | Tris, NaCl, DTT, azide, MgCl2, CoA, GlcN-1-PO4, UDP-GlcNAc, ammonium sulfate, CoCl2, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |