2OI2
Streptococcus pneumoniae Mevalonate Kinase in Complex with Diphosphomevalonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.597, 101.597, 83.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.890 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.890 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.271 |
| Number of reflections | 16144 | |
| Completeness [%] | 92.0 | 81.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | HEPES, KCl, DTT, AMPPNP, MgCl2, Diphosphomevalonate, PEG, formate, pH 7.5, SITTING DROP UNDER OIL, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
