2OHW
Crystal structure of the YueI protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.895, 39.443, 48.778 |
| Unit cell angles | 89.88, 108.05, 110.26 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.080 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.123 | 0.577 |
| Total number of observations | 15478 | |
| Number of reflections | 42599 | |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 94.3 | 86.9 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Tris-HCl pH 7.5, 28% PEG 1000, VAPOR DIFFUSION, temperature 294K |
