2OHF
Crystal structure of human OLA1 in complex with AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9764 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 141.253, 159.831, 55.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Entries 1JAL and 1NI3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.438 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 17590 | |
| <I/σ(I)> | 16.3 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 12% PEG 3350, 50 mM TRIS/HCl, 10 mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
