2OHE
Structural and mutational analysis of tRNA-Intron splicing endonuclease from Thermoplasma acidophilum DSM 1728
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.906, 46.093, 58.001 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 17.790 - 2.700 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 35520 | |
| <I/σ(I)> | 4.44 | |
| Completeness [%] | 98.0 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 2.4M NaCl, 0.1M Na/K phosphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
