2OH7
The Crystal Structure of Cypovirus Polyhedra containing the Human ZIP-kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-08-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.99830 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 102.742, 102.742, 102.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.800 - 2.450 |
| R-factor | 0.122 |
| Rwork | 0.112 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.128 | 0.348 |
| Number of reflections | 6770 | |
| <I/σ(I)> | 8.2 | 3.7 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 8.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 300 | The crystals used to determine this structure were directly purified from cells, in vivo crystallization in the cytoplasm of the cell, temperature 300K |
